Fibroblast Culture

Collagen is one of the fundamental building blocks responsible for the skin’s firmness, elasticity, youthful appearance, and radiance. With aging, collagen production gradually decreases, and for many years this loss has been addressed mainly through external treatments.

Fibroblast culture is a cellular-based treatment designed to stimulate the body’s own collagen production. In this method, fibroblast cells are isolated from a small skin sample taken from the patient, expanded under laboratory conditions, and later injected into areas requiring rejuvenation.

Procedure Process

During the procedure, a 3–10 mm skin sample is obtained under local anesthesia from an area that is relatively protected from sun exposure, most commonly behind the ear. This tissue sample is sent to a laboratory together with approximately 60 cc of the patient’s blood.

The laboratory phase takes about 4–6 weeks, during which fibroblast cells are cultured and prepared for injection. The injection technique is similar to dermal filler applications.
• Tissue harvesting: Performed once
• Injection sessions: Typically performed in three sessions

Clinical effects usually begin to appear after the second injection, with collagen production gradually increasing and continuing for up to one year.

Our Clinical Approach

Although fibroblast culture has been applied in our clinic for many years, the clinical outcomes and overall patient satisfaction did not meet our expectations. For this reason, it is not routinely preferred in our current practice. Instead, we focus on more advanced regenerative treatments that offer more predictable results, higher efficacy, and improved patient comfort.